DMEM, or Dulbecco’s Modified Eagle Medium is a widely used modification to Harry Eagle’s Minimal Essential Media (MEM). It contains four times the amount of vitamins and amino acids, increased glucose, and additional supplements such as iron. The original recipe includes 1000 mg/L of glucose, but different varieties now exist containing up to 4500 mg/L. Depending on the application, amounts of other components may also differ, such as sodium pyruvate, sodium bicarbonate, L-glutamine, HEPES, and phenol red. When sodium bicarbonate used as buffer system (3.7g/L), it requires a 5 to 10% CO₂ environment to maintain physiological pH. First developed to grow mouse embryonic cells, DMEM is now extensively used in culturing a wide variety of mammalian cell types and cell lines. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells.

Recommended use :

• Respect storage conditions of the product
• Do not use the product after its expiry date
• Store product in an area protected from light (not necessary for saline solutions).
• Manipulate the product in aseptic conditions (e.g. : under laminar air flow)
• Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. : gloves, mask,
  hygiene cap, overall…)
• The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or
  veterinary applications.

Uses :

Supplements, such as antibiotics, should be added as sterile supplements to the medium. Storage
conditions and shelf-life of supplemented products will be affected by the nature of the supplements.

Signs of deterioration :

Medium should be clear and free of particulate and flocculent material.

Do not use this medium if it is cloudy or contains precipitate.
Other evidence of deterioration may include color change or degradation of physical or performance
characteristics.