Ham’s F-10 was first developed by R. G. Ham (1962) for supporting single-cell growth of a diploid Chinese hamster ovary (CHO) cell line. This medium was found to be superior to an existing medium, F7, for both serum-free growth of CHO and for low-serum requirements of other mammalian cell lines. The key features include a higher amount of arginine, low tryptophan, proline and a variety of other nutrients such as thiamine, hypoxanthine and zinc. Depending on the application, commercial formulations of Ham’s F-10 may come with or without serum supplementation. Ham’s F-10 is known not only to support primary explants of chicken, rabbit and rat tissues, but also the growth of White Blood Cells (WBC’s) for chromosomal analysis and the growth of human diploid cells. F10 Nutrient Mixture (Ham’s) provides greater reliability and improved consistency in mammalian cell culture resulting in better overall efficiency.

Recommended use :
– Respect storage conditions of the product
– Do not use the product after its expiry date
– Store product in an area protected from light (not necessary for saline solutions).
– Manipulate the product in aseptic conditions (e.g. : under laminar air flow)
– Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
– The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or veterinary applications.

Utilisation :
Supplements, such as antibiotics, should be added as sterile supplements to the medium.
Storage conditions and shelf-life of supplemented product will be affected by the nature of the supplements.

Indications of deterioration :
Medium should be clear and free of particulate and flocculent material. Do not use if medium is cloudy or contains precipitate.
Other evidence of deterioration may include color change or degradation of physical or performance characteristics.

Composition : Displayed on website; also available on request